Part:BBa_I716029:Design

mgrB - rbs and stops at ATG

Assembly Compatibility:

• 10
  COMPATIBLE WITH RFC[10]
• 12
  COMPATIBLE WITH RFC[12]
• 21
  COMPATIBLE WITH RFC[21]
• 23
  COMPATIBLE WITH RFC[23]
• 25
  COMPATIBLE WITH RFC[25]
• 1000
  COMPATIBLE WITH RFC[1000]

Design Notes

This part is built in Biobrick version 2.0. In this format, parts are flanked by BglII and BamHI restriction sites.

making basic biobrick I716029 - Mg2+ repressed promoter (p-mgrB)

[pcr] AY021/23 onto MG1655 genome   (550 bp, BglII/XhoI)
put it into pBca9145
you get I716029

mgrB attempt by yu (550 bp)
AY021 (f)  catggAGATCTtgagagtaagaacctgtcgg 
AY023 (r2) gtacaCTCGAGttaGGATCCcgaaactttttcactccaatc

Source

K12 MG1655